Laboratory

Dr. Pilsner maintains an active research laboratory consisting of 1,500 sq. ft. of state-of-the-art facilities.  Dr. Pilsner's laboratory is custom designed specifically to process and analyze a wide variety of biologic samples for global and gene-specific epigenetic studies.  The laboratory is subdivided into four sections:

1) Main laboratory for real-time PCR and MassARRAY epigenetic analyses,
2) Positive pressurized amplicon-free room for pre-PCR set-up,
3) Office/break-room space for students, technicians and lab meetings
4) Equipment room containing 35 sq. ft. controlled-environment room and autoclave.

Dr. Pilsner's major equipment:

  • MassARRAY for genotype and epigenetic analyses (Sequenom)
    • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
    • 384-well format for high-throughput analyses
    • Targeted DNA methylation analyses up to 600 bp
    • Multiplexing SNP genotype analyses (up to 40 SNPs/sample)
    • Somatic mutational profiling
  • Nanodispensor (Sequenom)
    • Robotic pipetting system transfers nanoliter volumes of samples to SpectroCHIP arrays
  • Two 384-well Mastercyclers thermalcyclers (Eppendorf)
  • Two 96-well MyCyclers thermalcyclers (Bio-Rad)
  • 96-well StepONE Plus Real-time thermalcycler (Applied Biosystems)
  • epMotion robotic pipetting system (Eppendorf)
  • Refrigerated table top centrifuge 5810R (Eppendorf)
  • Gel Documentation Imaging Station with thermal printer (BioDoc)
  • NanoDrop 2000 (ThermoScientific)
  • Two Class II biosafety cabinets (Baker)
  • 35 sq ft controlled environment room
  • Autoclave